Cover of the e-book Vitamins in Functional Medicine: biochemistry, doses, active forms and clinical protocols.Coming soon

PDF e-book · 119 pages · Dr. Estela Reginatto

Vitamins in
Functional Medicine

"A normal blood count does not rule out deficiency." In a patient with diabetes who takes metformin and omeprazole, tingling in the feet and forgetfulness with a B12 of 230 pg/mL, in the gray zone, may be attributed to the diabetes alone, until elevated homocysteine and methylmalonic acid show that the vitamin is lacking. This e-book teaches you to see this subclinical zone: biomarkers with functional targets, the active form and dose of each vitamin, 100 sample formulas and 14 annotated clinical cases.

  • Monographs on the 13 vitamins and 18 vitamin-like factors: active form, dose, interactions and evidence
  • Biomarkers with cutoffs and functional targets, and the vitamins each medication depletes
  • 100 sample formulas and 14 annotated clinical cases, from suspicion to reassessment

The English edition is coming soon

Tap "Notify me" and send the ready-made WhatsApp message: we will let you know when the English edition is out.
Intended for health professionals. Patients and the general public can also buy it.

What almost no one tells you
about vitamins.

Vitamins are often treated as something simple: a multivitamin, a D3 capsule, a B complex "for energy". These are six points the e-book takes apart, each with the source for what is claimed and the module where the topic is covered in detail.

  • 01Modules 12 and 23

    "Excess vitamins are flushed out in the urine"

    Only some of them. A, D, E and K accumulate in the body and, even among those excreted in the urine, excess B6 causes sensory neuropathy: tingling in the hands and feet and unsteadiness when walking in the dark. In one of the e-book's cases, a B complex "for energy", a magnesium supplement with B6 and a hair formula add up to more than 100 mg/day of pyridoxine. The limit for long-term use of B6 is 100 mg/day according to the Food and Nutrition Board and, since 2023, 12 mg/day according to EFSA.

  • 02Module 19

    "My medication has nothing to do with vitamins"

    It does. Metformin and omeprazole lower B12; oral contraceptives, B6, B12, folate and B2; diuretics, thiamine and magnesium; statins, coenzyme Q10; anticonvulsants, vitamin D, folate and biotin. The e-book provides the table of depletions by medication, with the mechanism and the course of action, and the question it proposes for every anamnesis: "which vitamin does this medication deplete?"

  • 03Modules 14 and 15

    "Folic acid fixes anemia"

    It can mask the problem. At high doses, folic acid corrects the anemia caused by a lack of B12, but not the neurological damage, which keeps progressing and can become irreversible. And the nerve symptoms (tingling, loss of balance, changes in memory and mood) can appear before the anemia. That is why the e-book calls for ruling out B12 deficiency before treating a macrocytic anemia with folic acid.

  • 04Module 4

    "I take vitamin D and my level won't go up"

    Magnesium is a cofactor of the enzymes that activate vitamin D. Patients whose 25(OH)D "won't go up" on an adequate dose often have a low magnesium intake, malabsorption or obesity, or take the capsule without a meal containing fat. And the way it is taken matters: sporadic megadoses, of 300,000 to 500,000 IU once a year or every three months, were associated with more falls and fractures in some studies. The e-book provides the target range, the correction regimen and what to monitor.

  • 05Module 16

    "If it's called a vitamin, it's a vitamin"

    Not always. "Vitamin B15" (pangamic acid) and "vitamin B17" (amygdalin, a glycoside that releases cyanide) are not vitamins and have no demonstrated safe use. K3, synthetic menadione, causes hemolysis and should not be used in humans. And PABA, once called "vitamin Bx", is not a vitamin for humans. The e-book provides the table of current and obsolete nomenclature, from B4 to vitamin U.

  • 06Modules 7 and 20

    "Intravenous vitamin C is just a higher dose"

    It is a different effect. By mouth, absorption saturates and plasma levels rarely exceed about 220 µmol/L; intravenously, the concentration can be up to 70 times higher and, in this millimolar range, ascorbate starts to generate hydrogen peroxide, a pharmacological effect different from the nutritional one. That is why a high-dose infusion requires measuring G6PD and assessing kidney function beforehand: hemolysis and oxalate nephropathy are the serious complications described.

The e-book is not a menu for self-medication. The choice of vitamin, form, dose and duration of use depends on individual assessment, lab tests and current medications, and is the responsibility of a licensed professional, within the rules of their professional board. What it delivers is the complete reasoning: not only which vitamin is low, but why it became low, because the cause defines the form, the route and the duration of treatment.

From biochemistry to protocol,
with the limits of each vitamin.

The foundations open the e-book: what a vitamin is, vitamers, how deficiency sets in, reference values and the difference between the optimal range and minimum sufficiency. Then come the three parts for day-to-day reference.

Clinical pearl

Homocysteine is the "thermometer" of methylation: a single, accessible test that integrates folate, B12, B6 and B2. When it is above 9 to 10 µmol/L, investigate the four vitamins, kidney and thyroid function and the MTHFR polymorphism before treating with folic acid alone.

How the boxes appear in the e-book (module 17)
01
Part 2 · modules 3 to 16

Monographs: the 13 vitamins and 18 vitamin-like factors

Each vitamin in a standardized monograph: what it is, mechanism of action, indications, usual adult dose, how to use, contraindications and interactions and the level of evidence.

  • Vitamers and active forms, such as cholecalciferol, P5P, 5-MTHF, methylcobalamin, benfotiamine and MK-7, and when the form makes a difference
  • Absorption, transport, enzymatic functions, food sources with amounts per serving and the assessment of each vitamin
  • What the major trials showed, such as VITAL, SELECT, AREDS2, CSPPT, VITACOG and HPS2-THRIVE
  • Choline, betaine, carnitine, inositol, coenzyme Q10, lipoic acid, PQQ, carotenoids and flavonoids
  • Current and obsolete nomenclature, from "vitamin B4" to "vitamin U", and the false vitamins
02
Part 3 · modules 17 to 23

Assessment, protocols, 100 formulas and 14 cases

The lab tests that reveal deficiency before damage sets in and the strategies for the most common conditions in functional practice.

  • The best marker for each vitamin, with cutoffs, functional targets and interfering factors, and the panels by clinical goal
  • The methylation cycle, homocysteine and the MTHFR polymorphism, with the protocol for high homocysteine
  • The vitamins each long-term medication depletes, with the mechanism and the course of action
  • Protocols by indication: fatigue, immunity, bones, neuropathy, cognition, migraine, skin and hair, thyroid, fertility and more, and the intramuscular and intravenous routes
  • Pregnancy, older adults, vegans, athletes and post-bariatric patients, the 100 sample formulas and the 14 annotated cases
03
Part 4 · modules 24 to 28

Safety: what not to combine and how far to go

The signs of excess, the interactions and the limits that separate a therapeutic dose from toxicity.

  • When each vitamin becomes toxic and how excess shows up, from vitamin D hypercalcemia to B6 neuropathy
  • Interactions with warfarin, levodopa, methotrexate, phenytoin, statins, levothyroxine and others, with the course of action for each one
  • Biotin, which distorts thyroid, hormone and troponin tests, and how long to stop it before the sample is collected
  • Quick prescribing guide: forms and equivalences, maintenance and correction doses, upper limit and monitoring
  • Quality and labeling, frequently asked questions from patients and professionals and the glossary

119 pages in four parts.

From foundations to safety: what a vitamin is and how deficiency sets in, the monographs on each vitamin and on the vitamin-like factors, assessment with the protocols, the Formulary and the cases and, at the end, toxicity, interactions, quick guide, frequently asked questions and references. Every monograph carries its evidence label.

Part 1

Foundations

The concept of a vitamin, classification, absorption and utilization, stages and causes of deficiency, reference values and safety limits.

  1. Mod. 1What vitamins are
  2. Mod. 2Deficiency, requirements and safety

Part 2

Vitamin Monographs

The 13 vitamins and the vitamin-like and bioactive factors: technical profile, metabolism, functions, food sources, assessment, evidence and toxicity.

  1. Mod. 3Vitamin A and carotenoids
  2. Mod. 4Vitamin D
  3. Mod. 5Vitamin E
  4. Mod. 6Vitamin K
  5. Mod. 7Vitamin C
  6. Mod. 8Thiamine (vitamin B1)
  7. Mod. 9Riboflavin (vitamin B2)
  8. Mod. 10Niacin (vitamin B3) and NAD+ precursors
  9. Mod. 11Pantothenic acid (vitamin B5)
  10. Mod. 12Vitamin B6
  11. Mod. 13Biotin (vitamin B7)
  12. Mod. 14Folate (vitamin B9)
  13. Mod. 15Vitamin B12 (cobalamin)
  14. Mod. 16Vitamin-like factors

Part 3

Assessment and Clinical Protocols

Biomarkers and functional ranges, methylation, drug-induced depletions, protocols by indication, injectable routes, life stages, 100 formulas and 14 cases.

  1. Mod. 17Laboratory assessment of vitamin status
  2. Mod. 18Methylation cycle and homocysteine
  3. Mod. 19Drug-induced vitamin depletion
  4. Mod. 20Protocols by clinical indication
  5. Mod. 21Life stages and special populations
  6. Mod. 22Sample formulary
  7. Mod. 23Annotated clinical cases

Part 4

Safety and Clinical Practice

Toxicity, interactions, laboratory interference, quick prescribing guide, frequently asked questions, glossary and references.

  1. Mod. 24Safety: toxicity, interactions and quality
  2. Mod. 25Quick prescribing guide
  3. Mod. 26Frequently asked questions
  4. Mod. 27Glossary
  5. Mod. 28Scientific references

Evidence labels · Table 2

[MA]
Meta-analysis or systematic review of clinical trials
[ECR]
Published randomized controlled trial
[EC]
Smaller, open-label or observational clinical study
[PC]
Preclinical evidence (cell or animal studies)
[CS]
Consensus or guideline of a scientific society or regulatory agency

Written for those who prescribe and for those who take them.

The e-book is intended for health professionals, but patients and the general public can also buy it. The professional finds standardized monographs, levels of evidence, biomarkers with cutoffs, interaction tables and protocols by indication; the patient finds, throughout the text, the "For the patient" boxes, with explanations in plain language, and arrives at the appointment knowing what to ask.

Health professionals

In functional and integrative medicine and nutrition, within the scope of their legal competencies, who assess, prescribe or advise on vitamins in clinical practice.

  • Monographs in the same template for the 13 vitamins and 18 vitamin-like factors, with the active form, the usual dose, the interactions and the level of evidence
  • Biomarkers with cutoffs and functional targets, panels by clinical goal and unit conversion
  • Protocols by indication, 100 formulas with dosing schedules as a starting point for individualizing and 14 annotated cases
  • The tables on depletion by medication, interactions, signs of excess and upper limits

Patients and the general public

Who take or are considering taking vitamins and want to understand what they take, before starting or during treatment.

  • The 22 "For the patient" boxes, in plain language, throughout the e-book
  • Why vitamins are not always harmless and which ones accumulate in the body
  • Which everyday medications can lower vitamin levels and what to tell your health professional
  • The most common questions from people who take vitamins, answered in Module 26

Intended for health professionals. For patients and the general public, the e-book is a way to understand the use of vitamins: it is not a prescription, it does not replace a consultation with a licensed professional, and formulas and doses should not be used on your own.

The complete e-book
coming soon in English.

The English edition is in preparation. Leave your request and we will message you on WhatsApp when it launches.

Cover of the e-book Vitamins in Functional Medicine: biochemistry, doses, active forms and clinical protocols.Coming soon

AuthorDr. Estela Reginatto@nutri.estela.reginattoNovember 2026 edition

What you get
  • 119-page PDF e-book with 28 modules in 4 parts
  • Monographs on the 13 vitamins and 18 vitamin-like and bioactive factors: what it is, mechanism, indications, dose, how to use, contraindications and evidence
  • Biomarkers with cutoffs and functional targets, the methylation cycle and drug-induced depletions
  • Protocols by indication: fatigue, immunity, bones, neuropathy, cognition, migraine, skin, thyroid, fertility and sports
  • Formulary with 100 sample formulas and 14 annotated clinical cases
  • Safety: signs of excess, interactions, a quick prescribing guide with the upper limits and frequently asked questions
  • 93 tables, 11 figures, a glossary and 33 scientific references
PDF pages
119
sample formulas
100
annotated clinical cases
14
tables
93
scientific references
33

Every monograph carries its level of evidence ([MA] [ECR] [EC] [PC] [CS]), from meta-analysis to scientific society guideline, and the doses match the ranges described in clinical studies, guidelines and integrative practice for adults.

The English edition is coming soon

Tap "Notify me" and send the ready-made WhatsApp message: we will let you know when the English edition is out.

Frequently asked
questions

If yours is not here, write to contato@basefuncional.com.

Dr. Estela Reginatto
Partner

Dr. Estela Reginatto

@nutri.estela.reginatto

Clinical nutritionist, specialist in functional medicine and nutrition.

She has a strong focus on pregnancy, weight loss and clinical nutrition for disease. In pregnancy, she nourishes the mother's health and the baby's development. In weight loss, she goes beyond the scale and treats the cause: metabolism, hormones and the gut. In established diseases, she makes food part of the treatment. In hormonal and thyroid health, she combines functional nutrition with herbal medicine.

She holds a postgraduate degree in Nutritional Adequacy and Maintenance of Endocrine Homeostasis: Prevention and Treatment of Age-Related Diseases, with Dr. Lair Ribeiro. She also holds postgraduate degrees in Phytotherapy and in Food Quality Management.

The active form and the right dose,
and the test that shows what is missing.

Notify me

119 pages, 100 sample formulas and 14 clinical cases, coming soon in English.